Biology Program Facilities
This lab is equipped with four working computer stations; a large fume hood; multiple aquaria for housing live aquatic organisms; and a large collection of preserved animal specimens from all the major taxanomic phyla that allow Animal Biology students to see first-hand the organisms they have been studying in class.
Animal Physiology students are able to conduct experiments with human and animal organ systems to gain a better understanding of how these systems work. Students utilize sophisticated, computer based data acquisition equipment that allows them to record and analyze physiological functioning in living nerve, muscle, cardiac, urinary and respiratory systems.
Human Anatomy students are given the unique opportunity to participate in cadaver dissection. John Brown University is one of only two universities in the state of Arkansas that makes cadaver dissection available to undergraduate students. This opportunity gives students advanced preparation for the training they will encounter during their graduate studies.
Animal Care Facility & Darkroom (BSH 010)
This facility is primarily used for housing animals used for teaching labs and research projects. One of the three animal cage rooms has been modified in this facility to function as a dedicated darkroom. It is used for loading film cassettes with X-ray film and chemiluminescent (or radioactive) blots and gels. It houses an X-ray film processor, which is used to develop exposed X-ray film.
Tissue Culture Lab (BSH 125)
This lab is used for maintaining mammalian cell lines in culture, doing sterile transfers of these cell lines, and conducting gene transfer experiments involving these cell lines. Equipment in this lab includes a refrigerator, BioSafety cabinet, CO2 incubators (single and double-stacked units), inverted light microscope, water bath, clinical centrifuge, vortexer and cart.
Research Lab (BSH 126)
This lab is used for performing a wide variety of techniques in Molecular Biology and Biochemistry, such as the construction, purification, and modification of recombinant plasmid DNAs, electroporation of bacterial and mammalian cells, gel electrophoresis and analysis of reporter gene activity in extracts of mammalian cells. This laboratory contains a -20° freezer, refrigerator, incubator, electrophoresis equipment (horizontal and vertical gels, DNA sequencing), fraction collector, thermal cycler (for PCR), water baths, luminometer, electroporation apparatus, dry bath incubators, sonicator and computer.
Radioisotope Lab (BSH 128)
This lab is currently used for measuring DNA concentrations, electroblotting gels, drying down gels and photodocumentation of gels. Equipment items in this room include: a fumehood, a UV/visible spectrophotometer, two semidry electroblotters, a gel dryer, vacuum pump, combined benchtop liquid scintillation counter and luminometer, and a photodocumentation system equipped with a digital camera, computer, UV transilluminator and white light converter.
Advanced Biology Lab (BSH 129)
This lab is used primarily for procedures that require centrifugation of large volumes of liquid. The principal equipment in this room includes a floor model refrigerated centrifuge and a benchtop refrigerated clinical centrifuge, each with several rotors. Also included in this room are four computers, three camera systems for phase contrast microscopes, three fume hoods, a floor model refrigerated incubator, numerous benchtop incubators and water baths, a BioSafety cabinet, power supply (for gel electrophoresis), double wide refrigerator with sliding glass doors and a reverse osmosis water system. This lab is also used for teaching laboratory sections of advanced biology courses.
Equipment Room (BSH 131)
This room houses larger pieces of equipment such as an ultracentrifuge (for density gradient centrifugation), a baking oven (for drying/baking glassware), a -80° freezer (for storing specific reagents and bacterial strains), a shaker water bath and shaker incubator (for growing liquid bacterial cultures), a SpeedVac Concentrator System (for drying down pelleted nucleic acids and concentrating solutions), a liquid nitrogen-based cryogenic storage system (for storing frozen mammalian cells and tissues) and a smaller Dewar flask for storing liquid nitrogen used for snap freezing samples.
The Greenhouse is used by students in Introductory and Advanced Plant Biology. Students are exposed to a wide variety of plant species grown in the greenhouse. The opportunity to dissect and study the plants gives students a better understanding of the workings of the organism. Students in Advanced Plant Biology have the opportunity to conduct their own independent research in the greenhouse. Students have more freedom to explore their interests in this area and can apply many of the conceptual things they have learned in the classroom as they conduct their research.